Hamilton Polymeric HPLC Columns: Applications in Ion Exchange and Exclusion
Explore Hamilton PRP polymeric HPLC columns for robust ion exchange, carbohydrate separation, and extreme chemical resistance.
Hamilton Polymeric HPLC Columns: Applications in Ion Exchange and Exclusion Abstract Polymeric HPLC stationary phases offer distinct advantages over silica-based packings, including 100% pH stability (pH 0 to 14) and resistance to chemical dissolution. This article highlights Hamilton PRP polymeric columns for ion exchange and carbohydrate analysis. Introduction While silica-based columns dominate reversed-phase chromatography, certain analytical applications—such as strong acid/base mobile phases or specialized ion chromatography—demand polymeric packings [1]. Hamilton Company is a global leader in polymer-based HPLC columns. ROWELL provides analytical laboratories with genuine Hamilton chromatography products. Hamilton PRP Column Portfolio | Series | Matrix / Chemistry | Primary Analytical Application | | :--- | :--- | :--- | | Hamilton PRP-X100 | Polystyrene-divinylbenzene (PS-DVB) anion exchanger | Inorganic anions, organic acids, carbohydrates | | Hamilton PRP-X200 | PS-DVB cation exchanger | Transition metals, organic bases | | Hamilton PRP-1 | Reversed-phase polymeric resin | Extreme pH mobile phases, pharmaceutical stress testing | 1. 100% pH Stability (pH 0 to 14) Unlike silica, Hamilton polymer resins endure harsh cleaning procedures using concentrated acids or bases, ensuring permanent column regeneration. 2. Selectivity for Ionic Species Functionalized polymeric resins provide precise ion exchange selectivities for inorganic ions and organic acids. Practical Recommendations - Equilibration : Allow sufficient column equilibration time when switching ionic strength or pH conditions. - Temperature Control : Utilize column ovens for reproducible retention times in ion chromatography. Product Recommendations from ROWELL - Hamilton PRP Series Columns : Available v