Agilent Poroshell 120 Series Selection and Application Guide
An in-depth selection guide for Agilent Poroshell 120 superficially porous columns, delivering UHPLC efficiency at standard HPLC pressures.
Agilent Poroshell 120 Series Selection and Application Guide Abstract The Agilent Poroshell 120 family of superficially porous particle columns bridges the gap between conventional HPLC and ultra-high-pressure liquid chromatography (UHPLC). This guide reviews stationary phase variants, particle architecture benefits, and application strategies for analytical laboratories. Introduction Laboratory productivity relies heavily on achieving high-resolution separations quickly without overtaxing instrument pressure limits. Superficially porous particle (core-shell) technology revolutionized this domain by offering significantly reduced mass transfer resistance compared to fully porous particles [1]. As an authorized distributor of leading chromatography brands, ROWELL supplies the complete Agilent Poroshell 120 portfolio to global analytical facilities. Poroshell 120 Stationary Phase Variants The Poroshell 120 line offers diverse chemistries tailored to specific separation challenges: | Stationary Phase | Chemistry & Key Features | Recommended Application | | :--- | :--- | :--- | | Poroshell 120 EC-C18 | General-purpose C18 with high carbon load and endcapping. | Pharmaceuticals, small molecules, broad pH range. | | Poroshell 120 SB-C18 | Stable-bond C18 without endcapping; optimized for low pH. | Peptides, organic acids, acidic pharmaceuticals. | | Poroshell 120 Phenyl-Hexal | Provides pi-pi interactions for aromatic and structural isomers. | Aromatic hydrocarbons, complex natural extracts. | | Poroshell 120 HILIC | Designed for hydrophilic interaction liquid chromatography. | Polar metabolites, polar drugs, carbohydrates. | 1. Particle Architecture and Efficiency Poroshell 120 columns feature a 2.7 µm particle with a 1.7 µm solid core and a 0.5 µm porous shell. This geometr