Therapeutic drug monitoring of six contraindicated/co-administered drugs by simple and green RP-HPLC-PDA; application to spiked human plasma.

This study focuses on therapeutic drug monitoring of six contraindicated/co-administered drugs by simple and green rp-hplc-pda; application to spiked human plasma.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the clinical field. Therapeutic drug monitoring is an important clinical testing of the drugs to monitor their concentrations in plasma in order to guarantee their optimal impact, and to avoid any side effects resulting from drug-drug interactions. A green reversed-phase high-performance liquid chromatographic method using a photodiode array detector (RP-HPLC-PDA) was developed for... Research Background and Significance Therapeutic drug monitoring (TDM) plays a crucial role in optimizing pharmacotherapy by measuring drug concentrations in biological matrices, primarily plasma, to ensure efficacy while minimizing toxicity. In clinical settings, the complexity increases when multiple drugs with potential contraindications or co-administration scenarios are involved, as drug-drug interactions can alter pharmacokinetics. The study by Hesham et al. (2024) emphasizes the need for robust analytical methods capable of simultaneously quantifying six such drugs in human plasma. This is significant due to the clinical necessity of monitoring these drugs to tailor individual patient therapy, avoid overdosing, and prevent adverse effects. Employing green analytical techniques aligns with the growing demand for environmentally sustainable laboratory practices, reducing hazardous solvent usage and waste generation. Experimental Design and Methodology The study developed a reversed-phase high-performance liquid chromatographic method using a photodiode array detector (RP-HPLC-PDA) to quantify six contraindicate