Therapeutic drug monitoring of mycophenolic acid and azole antifungals on two distinct LC-MS/MS instruments.

This study focuses on therapeutic drug monitoring of mycophenolic acid and azole antifungals on two distinct lc-ms/ms instruments.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the clinical field. As an active metabolite of a commonly prescribed immunosuppressant, mycophenolic acid (MPA) levels are often monitored to prevent organ rejection following a transplant. Triazoles are often prescribed for treatment of invasive fungal infections in immunocompromised patients. Due to the variability in individual pharmacokinetics and drug-drug interactions, therapeutic drug monitoring is...

This study focuses on therapeutic drug monitoring of mycophenolic acid and azole antifungals on two distinct lc-ms/ms instruments.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the clinical field. As an active metabolite of a commonly prescribed immunosuppressant, mycophenolic acid (MPA) levels are often monitored to prevent organ rejection following a transplant. Triazoles are often prescribed for treatment of invasive fungal infections in immunocompromised patients. Due to the variability in individual pharmacokinetics and drug-drug interactions, therapeutic drug monitoring is... Research Background and Significance Therapeutic drug monitoring (TDM) plays an essential role in optimizing pharmacotherapy, especially for drugs with narrow therapeutic windows and significant interpatient variability. Mycophenolic acid (MPA), the active metabolite of mycophenolate mofetil, is widely used as an immunosuppressant in transplant patients to prevent organ rejection. Maintaining appropriate MPA plasma concentrations is critical to balancing efficacy and toxicity. Similarly, azole antifungals are critical agents for treating invasive fungal infections in immunocompromised patients, such as those undergoing transplantation or chemotherapy. Given the complex pharmacokinetics and frequent drug-drug interactions associated with these agents, precise and reliable monitoring is imperative to tailor dosing and improve clinical outcomes. This study addresses the analytical challenges encountered in clinical laboratories by employing validated high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) methods to quantify both MPA and azole antifungals. By comparing two distinct LC-MS/MS instr