Therapeutic drug monitoring of linezolid: HPLC-based assays for routine quantification of linezolid in human serum and cerebrospinal fluid.

This study focuses on therapeutic drug monitoring of linezolid: hplc-based assays for routine quantification of linezolid in human serum and cerebrospinal fluid.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the clinical field. Therapeutic drug monitoring (TDM) of linezolid can prevent over- and under-dosing in critically ill patients and can be crucial to successful antibiotic treatment. Quick and simple high-performance liquid chromatography (HPLC) assays for the detection of linezolid in human serum and cerebrospinal fluid (CSF) were developed in this study.... Research Background and Significance Therapeutic drug monitoring (TDM) plays a pivotal role in optimizing antibiotic therapy, especially for critically ill patients where pharmacokinetic variability can significantly affect drug efficacy and safety. Linezolid, an oxazolidinone antibiotic, is commonly used for treating multidrug-resistant Gram-positive infections, including those involving the central nervous system. Due to its narrow therapeutic index and variable pharmacokinetics, routine monitoring of linezolid concentrations in biological matrices such as human serum and cerebrospinal fluid (CSF) is essential to avoid sub-therapeutic dosing or toxicity. The study by Günther et al. (2023) addresses the clinical need for rapid, reliable, and straightforward high-performance liquid chromatography (HPLC) assays that can be implemented in hospital laboratories for routine quantification of linezolid. This research advances the analytical capabilities by developing validated HPLC methods tailored to clinical matrices, thereby supporting personalized dosing strategies and improving patient outcomes. Experimental Design and Methodology The authors