Targeted and Non-Targeted HPLC Analysis of Coffee-Based Products as Effective Tools for Evaluating the Coffee Authenticity.

This study focuses on targeted and non-targeted hplc analysis of coffee-based products as effective tools for evaluating the coffee authenticity.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the chemical field. Coffee is a very popular beverage worldwide. However, its composition and characteristics are affected by a number of factors, such as geographical and botanical origin, harvesting and roasting conditions, and brewing method used. As coffee consumption rises, the demands on its high quality and authenticity naturally grows as well. Unfortunately,...

This study focuses on targeted and non-targeted hplc analysis of coffee-based products as effective tools for evaluating the coffee authenticity.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the chemical field. Coffee is a very popular beverage worldwide. However, its composition and characteristics are affected by a number of factors, such as geographical and botanical origin, harvesting and roasting conditions, and brewing method used. As coffee consumption rises, the demands on its high quality and authenticity naturally grows as well. Unfortunately,... Research Background and Significance Coffee, one of the most widely consumed beverages globally, possesses a complex chemical profile influenced by numerous variables such as geographical origin, botanical variety, harvesting practices, roasting conditions, and brewing techniques. These factors collectively impact the sensory attributes and chemical composition of coffee, which are critical determinants of quality and consumer acceptance. With the expansion of the coffee market and increasing consumer awareness, the authenticity and quality control of coffee products have become paramount. Adulteration and mislabeling pose significant challenges, necessitating robust analytical techniques to ensure product integrity. High-performance liquid chromatography (HPLC) serves as a powerful analytical tool in this context, enabling both targeted and non-targeted profiling of coffee constituents. This study by Klikarová and Česlová leverages HPLC methodologies to evaluate coffee authenticity, providing insights into how chemical fingerprinting can identify and authenticate coffee products effectively. Experimental Design and Methodology The study employs a d