Simultaneous and sensitive analysis of glyphosate, glufosinate, and their metabolites in surface water by HPLC-ICP-MS/MS.

This study focuses on simultaneous and sensitive analysis of glyphosate, glufosinate, and their metabolites in surface water by hplc-icp-ms/ms.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the environmental field. Organophosphorus pesticides such as glyphosate and glufosinate are used worldwide, and environmental regulatory values are being adopted in many countries due to their potential toxicity. In the present work, a pretreatment-free analytical method is established in which these two compounds with their metabolites are isolated from each other by anion-exchange... Research Background and Significance The widespread use of organophosphorus herbicides such as glyphosate and glufosinate has raised significant concerns regarding their environmental persistence and potential toxicity. These compounds, along with their metabolites, frequently contaminate surface waters, posing risks to aquatic ecosystems and human health. Regulatory agencies worldwide are increasingly implementing monitoring programs to control permissible levels of these pesticides in environmental matrices. However, the analytical quantification of glyphosate, glufosinate, and their metabolites presents substantial challenges due to their high polarity, low molecular weight, and lack of strong chromophores, which complicate detection by conventional chromatographic techniques. The study by Morimoto et al. addresses these challenges by developing a simultaneous and sensitive analytical method using high-performance liquid chromatography coupled with inductively coupled plasma tandem mass spectrometry (HPLC-ICP-MS/MS). This approach offers enhanced selectivity and sensitivity for phosphorus-containing analytes without the need for ex