Simple HPLC-UV Method for Therapeutic Drug Monitoring of 12 Antiepileptic Drugs and Their Main Metabolites in Human Plasma.

This study focuses on simple hplc-uv method for therapeutic drug monitoring of 12 antiepileptic drugs and their main metabolites in human plasma.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the clinical field. The objective of the present report was to develop and validate a simple, selective, and reproducible high-performance liquid chromatography method with UV detection suitable for routine therapeutic drug monitoring of the most commonly used antiepileptic drugs and some of their metabolites. Simple precipitation of plasma proteins with acetonitrile was used... Research Background and Significance Therapeutic drug monitoring (TDM) plays a crucial role in the clinical management of patients undergoing antiepileptic drug therapy. Given the narrow therapeutic windows and variable pharmacokinetics of many antiepileptic drugs (AEDs), maintaining optimal plasma concentrations is essential to ensure efficacy while minimizing toxicity. Traditional analytical methods for AED quantification often face challenges such as complexity, lengthy sample preparation, and limited multiplexing of drugs and metabolites. The study by Milosheska and Roškar (2023) addresses these limitations by developing a simple, selective, and reproducible HPLC-UV method capable of quantifying 12 commonly used AEDs and their main metabolites in human plasma. This approach is significant as it proposes a streamlined method that supports routine clinical monitoring without the need for highly sophisticated instrumentation. The simplicity of the sample preparation and the use of UV detection make this methodology accessible and cost-effective for many clinical laboratories. Experimental Design and Methodology The experimental approach c