Rapid and simultaneous purification of aflatoxin B1, zearalenone and deoxynivalenol using their monoclonal antibodies and magnetic nanoparticles.

This study focuses on rapid and simultaneous purification of aflatoxin b1, zearalenone and deoxynivalenol using their monoclonal antibodies and magnetic nanoparticles.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the biopharmaceutical field. To develop a new simple and simultaneous purification method for mycotoxins in feeds and grains, magnetic nanoparticles (MNPs) conjugated with monoclonal antibodies (mAbs) against mycotoxins were used to separate aflatoxin B1 (AFB... Research Background and Significance Mycotoxins such as aflatoxin B1 (AFB1), zearalenone (ZEN), and deoxynivalenol (DON) are toxic secondary metabolites produced by fungi that frequently contaminate agricultural commodities like grains and animal feeds. These mycotoxins pose significant health risks to humans and animals, including carcinogenic, estrogenic, and immunosuppressive effects. Consequently, accurate, rapid, and simultaneous detection and purification of these toxins are critical in food safety, feed quality assurance, and biopharmaceutical applications. Traditional purification methods tend to be time-consuming, often requiring separate steps for each toxin, limiting throughput and increasing cost. The study by Lee and Kang (2021) addresses these challenges by developing a novel, rapid purification strategy that leverages the specificity of monoclonal antibodies (mAbs) conjugated onto magnetic nanoparticles (MNPs) to selectively isolate multiple mycotoxins simultaneously. This approach aims to streamline sample preparation and improve analytical efficiency when coupled with high-performance liquid chromatography (HPLC), thereby enhancing the reliability and speed of mycotoxin monitoring in complex matrices. Experimental Des