Quantitative Analysis and Differential Evaluation of Radix Bupleuri Cultivated in Different Regions Based on HPLC-MS and GC-MS Combined with Multivariate Statistical Analysis.

This study focuses on quantitative analysis and differential evaluation of radix bupleuri cultivated in different regions based on hplc-ms and gc-ms combined with multivariate statistical analysis.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the environmental field. The quality of Radix Bupleuri is greatly affected by its growing environment. In this study, Radix Bupleuri samples that were harvested from seven different regions across northwest China were examined by high-performance liquid chromatography (HPLC) and gas chromatography (GC) coupled with mass spectrometry (MS) to reveal significant differences in quality... Research Background and Significance Radix Bupleuri, a traditional herbal medicine widely utilized for its pharmacological properties, exhibits significant variability in chemical composition depending on its geographical origin. The environmental factors influencing plant growth, such as soil composition, climate, and altitude, directly impact the quality and efficacy of the herbal material. Accurate quantitative analysis of Radix Bupleuri metabolites is thus critical not only for quality control but also for understanding environmental influences on medicinal plant biochemistry. This study addresses the need for robust analytical techniques capable of differentiating Radix Bupleuri samples from disparate cultivation regions. Employing a combination of high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) and gas chromatography-mass spectrometry (GC-MS) alongside multivariate statistical analysis, the research provides a comprehensive evaluation of regional differences. This is particularly significant in environmental applications where the correlation be