Quality and composition control of complex TCM preparations through a novel "Herbs-in vivo Compounds-Targets-Pathways" network methodology: The case of Lianhuaqingwen capsules.

This study focuses on quality and composition control of complex tcm preparations through a novel "herbs-in vivo compounds-targets-pathways" network methodology: the case of lianhuaqingwen capsules.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the chemical field. Lianhuaqingwen (LHQW) capsules, a Chinese patent medicine composed of 13 herbal ingredients, are widely used for respiratory diseases. However, the complex composition of LHQW poses challenges in assessing its quality and consistency. In this study, a comprehensive network of LHQW was constructed by integrating Digital RNA with pertUrbation of Genes... Research Background and Significance Traditional Chinese Medicine (TCM) formulations such as Lianhuaqingwen (LHQW) capsules are complex mixtures comprising multiple herbal components, which present significant challenges in quality control and compositional analysis. LHQW capsules, containing 13 distinct herbal ingredients, are widely prescribed for respiratory ailments, including viral infections. However, the multifaceted chemical makeup and the inherent variability of natural products complicate conventional quality assessment approaches. The study by Chen et al. (2025) addresses these challenges by integrating a novel "Herbs-in vivo Compounds-Targets-Pathways" network methodology with high-performance liquid chromatography (HPLC) to achieve comprehensive chemical profiling and quality control of LHQW. This approach is significant as it bridges in vivo pharmacodynamics with chemical analytics, providing a robust framework for ensuring consistency, efficacy, and safety of complex TCM formulations. Experimental Design and Methodology The researchers employed validated HPLC techniques to perfor