Preparation of Monoclonal Antibodies Specifically Reacting with the Trichothecene Mycotoxins Nivalenol and 15-Acetylnivalenol via the Introduction of a Linker Molecule into Its C-15 Position.

This study focuses on preparation of monoclonal antibodies specifically reacting with the trichothecene mycotoxins nivalenol and 15-acetylnivalenol via the introduction of a linker molecule into its c-15 position.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the biopharmaceutical field. Nivalenol (NIV) is a trichothecene mycotoxin that is more toxic than deoxynivalenol. It accumulates in grains due to infection with... Research Background and Significance Trichothecene mycotoxins, such as nivalenol (NIV) and its derivative 15-acetylnivalenol, pose significant health risks due to their potent toxicity and frequent contamination of cereal grains. NIV, in particular, is known to be more toxic than the commonly studied deoxynivalenol (DON), exacerbating the need for precise detection and quantification methods in food safety and biopharmaceutical contexts. Monoclonal antibodies (mAbs) that specifically recognize these toxins can facilitate sensitive immunoassays, but developing such antibodies requires careful antigen design. This study addresses the challenge by introducing a linker molecule into the C-15 position of NIV, enabling the generation of highly specific monoclonal antibodies. The application of high-performance liquid chromatography (HPLC) in this research serves a dual purpose: verifying the chemical modifications during antibody preparation and supporting the analytical characterization of resulting antibodies. This work is significant as it advances both immunochemical reagent development and analytical methodology for trichothecene mycotoxins, aligning with growing demands in the biopharmaceutical field for robust and reliable toxin detection. Experimental Design and Methodology The method