Mycotoxins in

This study focuses on mycotoxins in . The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the environmental field. Present research delves in the isolation, extraction and identification of mycotoxins from ten corn samples collected from the northern province of Pakistan. Average concentration of aflatoxin B1 and B2 by HP-TLC found in all corn samples was 27.87 and 1.35 μg/kg, respectively. Following HP-TLC, detoxification of the identified and isolated... Research Background and Significance Mycotoxins are toxic secondary metabolites produced by various fungi, notably Aspergillus, Fusarium, and Penicillium species. These compounds pose significant health risks to humans and animals, primarily through contaminated food and agricultural products. Corn, a staple crop worldwide, is highly susceptible to fungal contamination and consequent mycotoxin accumulation, especially aflatoxins B1 and B2, which are among the most potent carcinogens. Environmental monitoring of mycotoxins in foodstuffs is crucial for public health and safety. The research by Murad et al. (2022) focuses on detecting and quantifying mycotoxins in corn samples collected from northern Pakistan, emphasizing the environmental analysis aspect. The study leverages high-performance liquid chromatography (HPLC) techniques to overcome analytical challenges associated with the isolation, identification, and quantification of these toxins in complex matrices. This work is significant as it provides validated chromatographic methods to ensure accurate environmental monitoring and food safety compliance. Experimental Design and Methodology The study collected ten corn samples from the northern province of Pakistan to investigate the presence and concentra