Multivariate Analysis and Response Surface Modeling to Green Analytical Chemistry-Based RP-HPLC-PDA Method for Chromatographic Analysis of Vildagliptin and Remogliflozin Etabonate.

This study focuses on multivariate analysis and response surface modeling to green analytical chemistry-based rp-hplc-pda method for chromatographic analysis of vildagliptin and remogliflozin etabonate.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the environmental field. The fixed-dose combination (FDC) of vildagliptin (VDG) and remogliflozin etabonate (RGE) is used as antidiabetic medicine. Numerous reverse phase high-pressure liquid chromatographic (RP-HPLC) methods have been reported for the estimation of VDG and RGE using toxic organic solvents such as acetonitrile and methanol. These organic solvents are also hazardous to... Research Background and Significance The determination of pharmaceutical compounds in environmental matrices has become an increasingly important area of analytical research, especially given the rising concerns about pharmaceutical contaminants in water bodies and their ecological impacts. Vildagliptin (VDG) and Remogliflozin Etabonate (RGE) are antidiabetic agents commonly administered as a fixed-dose combination (FDC) to manage type 2 diabetes mellitus. Despite their clinical importance, conventional analytical methods for VDG and RGE quantification have typically relied on toxic organic solvents such as acetonitrile and methanol, which pose environmental and health hazards. This study addresses these challenges by developing a green analytical chemistry-based reversed-phase high-performance liquid chromatography (RP-HPLC) method coupled with photodiode array (PDA) detection. The use of multivariate analysis and response surface methodology (RSM) further enhances method optimization, aiming for improved efficiency, selectivity, and environmental sustainability. The signif