Lignin Analysis by HPLC and FTIR: Spectra Deconvolution and S/G Ratio Determination.

This study investigates lignin analysis by hplc and ftir: spectra deconvolution and s/g ratio determination.. The research utilizes high-performance liquid chromatography (HPLC) as the primary analytical technique to address specific challenges in pharmaceutical analysis. Fourier transform infrared spectroscopy (FTIR) is a simple nondestructive technique that allows the user to obtain quick and accurate information about the structure of the constituents of wood. Spectra deconvolution is a computational technique, complementary to FTIR analysis, which improves the resolution of overlapped or unobserved bands in the raw... Research Background and Significance Lignin is a complex and heterogeneous biopolymer found in the cell walls of plants, playing a crucial role in providing structural integrity. Its analysis is of high interest, particularly in pharmaceutical and biochemical research, due to its potential as a source of bioactive compounds and its relevance in biomass valorization. The syringyl/guaiacyl (S/G) ratio is a key parameter in lignin characterization, reflecting the relative abundance of these phenolic units and influencing lignin’s chemical reactivity and properties. Traditional analytical methods often face challenges in resolving overlapping signals from lignin’s complex structure. This study by Reyes-Rivera and Terrazas (2024) addresses these challenges by integrating High-Performance Liquid Chromatography (HPLC) with Fourier Transform Infrared Spectroscopy (FTIR) and advanced spectral deconvolution techniques. This combined approach aims to enhance the resolution and accuracy of lignin component analysis, providing robust data crucial for pharmaceutical applications where precise molecular characterization is paramount. Experimental Design and Methodolo