HPLC-MS/MS Method for Monitoring of L-Asparaginase Activity by Using Asparagine and Aspartic Acid Plasma Levels.

This study focuses on hplc-ms/ms method for monitoring of l-asparaginase activity by using asparagine and aspartic acid plasma levels.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the clinical field. The effectiveness of L-asparaginase and therefore the effectiveness of acute lymphoblastic leukemia treatment will be understood by determining the substrates of the enzyme, L-asparagine and L-glutamine. For this purpose, the high-performance liquid chromatography-tandem mass spectrometry method was developed and validated by analyzing the L-asparaginase substrates asparagine and glutamine and its... Research Background and Significance L-asparaginase is a critical enzyme widely used in the treatment of acute lymphoblastic leukemia (ALL), a common pediatric malignancy. The therapeutic efficacy of L-asparaginase relies on its ability to catalyze the hydrolysis of L-asparagine into L-aspartic acid and ammonia, thereby depleting circulating asparagine levels essential for leukemic cell survival. Monitoring L-asparaginase activity in patients is indispensable for optimizing treatment regimens, managing resistance, and minimizing adverse effects. Traditional methods for enzyme activity assessment often lack the sensitivity and specificity required for clinical decision-making. Therefore, the development of an accurate, robust, and sensitive analytical technique to quantify plasma levels of L-asparagine and its hydrolysis product L-aspartic acid is of paramount importance. The study by Kaplan et al. (2026) addresses this gap by utilizing high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS), offering enhanced selectivity and sensitivity in monitoring enzyme substrates and pro