HPLC-MS-based untargeted metabolomic analysis of differential plasma metabolites and their associated metabolic pathways in reproductively anosmic black porgy, Acanthopagrus schlegelii.

This study focuses on hplc-ms-based untargeted metabolomic analysis of differential plasma metabolites and their associated metabolic pathways in reproductively anosmic black porgy, acanthopagrus schlegelii.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the environmental field. Olfaction, a universal form of chemical communication, is a powerful channel for animals to obtain social and environmental cues. The mechanisms by which fish olfaction affects reproduction, breeding and disease control are not yet clear. To evaluate metabolites profiles, plasma from anosmic and control black porgy during reproduction was analyzed... Research Background and Significance Olfaction plays a pivotal role in fish biology, serving as a fundamental chemical communication mechanism that influences behaviors related to reproduction, social interaction, and environmental adaptation. Despite its importance, the biochemical pathways and metabolomic alterations underlying olfactory dysfunction in fish remain poorly understood. The black porgy (Acanthopagrus schlegelii) is a model species in aquaculture and environmental research, where reproductive health is critical for population sustainability. This study addresses a critical gap by applying high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) to perform untargeted metabolomic profiling on plasma samples from reproductively anosmic black porgy. The goal is to identify differential metabolites and associated metabolic pathways impacted by anosmia, elucidating the biochemical mechanisms that link olfactory impairment to reproductive dysfunction. This investigation holds significance for environmental monitoring and fishery management, providing in