HPLC methods for choloroquine determination in biological samples and pharmaceutical products.

This study focuses on hplc methods for choloroquine determination in biological samples and pharmaceutical products.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the pharmaceutical field. Review and assess pharmaceutical and clinical characteristics of chloroquine including high-performance liquid chromatography (HPLC)-based methods used to quantify the drug in pharmaceutical products and biological samples.... Research Background and Significance Chloroquine and its derivative, chloroquine phosphate, have long been utilized in the treatment of malaria and autoimmune diseases. More recently, interest in chloroquine and related compounds surged due to their potential antiviral properties. Precise quantification of chloroquine in biological matrices and pharmaceutical formulations is essential for pharmacokinetic studies, therapeutic drug monitoring, and quality control. High-performance liquid chromatography (HPLC) has emerged as a reliable technique to address the challenges posed by the complex matrices and low analyte concentrations typically encountered in these analyses. The reviewed study focuses on developing and validating HPLC methods tailored specifically for chloroquine determination, highlighting their pharmaceutical relevance and clinical applicability. Experimental Design and Methodology The study systematically reviews and assesses existing HPLC methods for chloroquine quantification in both biological samples (such as plasma or serum) and pharmaceutical products. The chromatographic techniques discussed predominantly employ reversed-phase HPLC, utilizing C18 columns due to their robustness and suitability for non-polar to moderately polar compounds like chloroquine. Method development e