HPLC and GC/MS analysis of

This study investigates hplc and gc/ms analysis of . The research utilizes high-performance liquid chromatography (HPLC) as the primary analytical technique to address specific challenges in pharmaceutical analysis. The methodology demonstrates the effectiveness of chromatographic separation for complex sample matrices. Results indicate the reliability and precision of the developed HPLC method for routine analysis. Research Background and Significance High-performance liquid chromatography (HPLC) remains a cornerstone analytical technique in pharmaceutical research and quality control due to its versatility, sensitivity, and robustness. The study by Kirillov et al. (2024) addresses the ongoing need for reliable chromatographic methods capable of separating and quantifying complex pharmaceutical compounds within challenging sample matrices. Given the increasing complexity of pharmaceutical formulations and the stringent regulatory requirements for purity and potency, developing validated analytical methods that combine accuracy with reproducibility is paramount. This research integrates HPLC with gas chromatography-mass spectrometry (GC/MS) to enhance analytical confidence, addressing both volatile and non-volatile components in pharmaceutical samples. The significance lies in demonstrating a practical, validated approach that ensures routine applicability, which is vital for pharmaceutical laboratories seeking dependable methods for batch release, stability testing, and impurity profiling. Experimental Design and Methodology The experimental approach focused primarily on developing an HPLC method tailored to separate and quantify target analytes within pharmaceutical matrices. The study employed reversed-phase chromatography as the principal mode of separation, leverag