HPLC analysis, genotoxic and antioxidant potential of Achillea millefolium L. and Chaerophyllum villosum Wall ex. Dc.

This study investigates hplc analysis, genotoxic and antioxidant potential of achillea millefolium l. and chaerophyllum villosum wall ex. dc.. The research utilizes high-performance liquid chromatography (HPLC) as the primary analytical technique to address specific challenges in pharmaceutical analysis. Methanolic and chloroformic extract of Achillea millefolium and Chaerophyllum villosum were evaluated for HPLC analysis, genotoxic and antioxidant potential.... Research Background and Significance Achillea millefolium L. (commonly known as yarrow) and Chaerophyllum villosum Wall ex. Dc. are two medicinal plants with a rich history of use in traditional medicine, primarily attributed to their bioactive compounds exhibiting antioxidant and genoprotective properties. The pharmaceutical interest in these plants stems from their potential to provide novel therapeutic agents that can mitigate oxidative stress and DNA damage—two critical factors implicated in various chronic diseases and carcinogenesis. The study under discussion employs high-performance liquid chromatography (HPLC) as the central analytical technique to characterize and quantify the phytochemical constituents extracted from these plants. HPLC offers the precision, sensitivity, and reproducibility necessary for pharmaceutical quality control and bioactivity assessment. This research is significant as it not only elucidates the chemical profile of these botanicals but also validates a robust HPLC methodology that can be applied for routine pharmaceutical analysis, ensuring consistent quality and efficacy of herbal formulations. Experimental Design and Methodology The experimental framework of the study involved the preparation of methanolic and chloroformic extracts from Achillea millefolium and Chaerophyllum