High Performance Liquid Chromatography (HPLC) with Fluorescence Detection for Quantification of Steroids in Clinical, Pharmaceutical, and Environmental Samples: A Review.

This study focuses on high performance liquid chromatography (hplc) with fluorescence detection for quantification of steroids in clinical, pharmaceutical, and environmental samples: a review.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the pharmaceutical field. Steroids are compounds widely available in nature and synthesized for therapeutic and medical purposes. Although several analytical techniques are available for the quantification of steroids, their analysis is challenging due to their low levels and complex matrices of the samples. The efficiency and quick separation of the HPLC combined with... Research Background and Significance Steroids are a diverse class of organic compounds that play vital roles in biological systems and are extensively utilized in pharmaceutical formulations. Their quantification in clinical, pharmaceutical, and environmental samples is crucial for therapeutic monitoring, quality control, and environmental safety assessments. However, steroid analysis is inherently challenging due to their low concentrations in complex biological matrices and the structural similarity among different steroid species. High-performance liquid chromatography (HPLC) coupled with fluorescence detection has emerged as a powerful analytical technique that combines high sensitivity, selectivity, and rapid separation capabilities. This review highlights the significance of HPLC-fluorescence methods in overcoming the analytical challenges posed by steroid quantification, emphasizing its relevance across clinical diagnostics, pharmaceutical quality control, and environmental monitoring. Experimental Design and Methodology The reviewed methodologies employ reversed-phase HPLC systems, typically