Green Approaches in High-Performance Liquid Chromatography for Sustainable Food Analysis: Advances, Challenges, and Regulatory Perspectives.

This study focuses on green approaches in high-performance liquid chromatography for sustainable food analysis: advances, challenges, and regulatory perspectives.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the food safety field. This review provides a comprehensive overview of the recent green innovations in high-performance liquid chromatography (HPLC) for sustainable food analysis. It outlines the principles of green analytical chemistry and examines advances such as eco-friendly solvent systems, miniaturized and energy-efficient instrumentation, and greener sample preparation techniques. Key applications include the analysis... Research Background and Significance High-performance liquid chromatography (HPLC) has long been a cornerstone analytical technique in food safety, enabling sensitive and selective detection of contaminants, residues, additives, and nutritional components. However, conventional HPLC methods often rely on hazardous organic solvents and energy-intensive instrumentation, contributing to environmental and safety concerns. The increasing global emphasis on sustainable practices has catalyzed the integration of green analytical chemistry principles into chromatographic methods. This study by Karageorgou et al. (2025) addresses the imperative need to develop eco-friendly HPLC approaches tailored for food analysis, balancing analytical rigor with environmental responsibility. The work is significant as it encapsulates advances in solvent reduction, solvent substitution, miniaturization, and energy efficiency while considering regulatory frameworks that guide method validation and acceptance in food safety laboratories. Experimental Design and Methodology The reviewed methodologies