Green Alternatives in Pharmaceutical and Bioanalytical Analysis of TDM Required Drugs: Procainamide.

This study focuses on green alternatives in pharmaceutical and bioanalytical analysis of tdm required drugs: procainamide.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the pharmaceutical field. In drug analysis, using non-hazardous solvents instead of the ones harmful to humans and the environment is a green strategy to protect analysts and environmental health.... Research Background and Significance Therapeutic drug monitoring (TDM) is a critical component in personalized medicine for optimizing drug dosage to achieve maximal therapeutic efficacy while minimizing toxicity. Procainamide, an antiarrhythmic agent, requires precise monitoring due to its narrow therapeutic window. Traditional analytical methods for procainamide quantification often rely on hazardous organic solvents that pose risks to both analysts and environmental health. The increasing regulatory and societal demands for sustainable laboratory practices have driven the development of greener analytical methodologies. This study addresses these concerns by exploring environmentally friendly alternatives in HPLC-based pharmaceutical and bioanalytical analysis of procainamide. By integrating green chemistry principles, the research aims to reduce the environmental footprint without compromising analytical performance, highlighting the dual benefit of safeguarding human health and the environment in routine pharmaceutical analysis. Experimental Design and Methodology The study employed high-performance liquid chromatography (HPLC) to develop and validate a green, reliable method for procainamide analysis. The experimental design focused on replacing hazardous solvents traditionally utilized in reversed-phase chromatographic separations wi