GC-MS and HPLC supported phytochemical analysis of watercress and the protective role against paraben toxicity.

This study investigates gc-ms and hplc supported phytochemical analysis of watercress and the protective role against paraben toxicity.. The research utilizes high-performance liquid chromatography (HPLC) as the primary analytical technique to address specific challenges in environmental analysis. In this study, the phytochemical content of Nasturtium officinale R. Br. (watercress) leaf extract (Noex) and its protective effects against paraben toxicity were investigated. GC-MS and HPLC analyses were performed to determine the phytochemical content. Paraben toxicity and protective properties of Noex were investigated with the Allium test, and 6... Research Background and Significance Phytochemicals derived from plants have gained considerable attention due to their diverse bioactive properties and potential protective effects against environmental toxins. Nasturtium officinale R. Br., commonly known as watercress, is a leafy aquatic plant rich in various secondary metabolites with antioxidant and detoxifying capabilities. The significance of this study lies in its dual analytical approach combining Gas Chromatography-Mass Spectrometry (GC-MS) and High-Performance Liquid Chromatography (HPLC) to comprehensively characterize the phytochemical profile of watercress leaf extract (Noex). Particularly, the research focuses on evaluating the protective effects of these phytochemicals against paraben toxicity, a class of widely used preservatives with known environmental and health concerns. By employing validated HPLC methods, the study addresses challenges of sensitivity and selectivity in environmental sample analysis, contributing valuable insights for environmental toxicology and phytochemical research. Experimental Design and Methodology The experimental framework integrat