Fosfomycin therapeutic drug monitoring in real-life: development and validation of a LC-MS/MS method on plasma samples.

This study focuses on fosfomycin therapeutic drug monitoring in real-life: development and validation of a lc-ms/ms method on plasma samples.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the clinical field. Individualization of fosfomycin dosing based on therapeutic drug monitoring (TDM) of plasma concentrations could reduce drug-related adverse events and improve clinical outcome in complex clinical conditions. Quantification of fosfomycin in plasma samples was performed by a rapid ultraperformance liquid chromatography mass spectrometry method. Sample preparation involved protein precipitation with [...

This study focuses on fosfomycin therapeutic drug monitoring in real-life: development and validation of a lc-ms/ms method on plasma samples.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the clinical field. Individualization of fosfomycin dosing based on therapeutic drug monitoring (TDM) of plasma concentrations could reduce drug-related adverse events and improve clinical outcome in complex clinical conditions. Quantification of fosfomycin in plasma samples was performed by a rapid ultraperformance liquid chromatography mass spectrometry method. Sample preparation involved protein precipitation with [... Research Background and Significance Fosfomycin is a broad-spectrum antibiotic increasingly used to treat multidrug-resistant bacterial infections, especially in complex clinical scenarios. Therapeutic drug monitoring (TDM) of fosfomycin plasma concentrations is critical to optimize dosing, minimize toxicity, and improve patient outcomes. However, clinical application of TDM requires robust, rapid, and reliable analytical methods capable of quantifying fosfomycin accurately in biological matrices. This study addresses this need by developing and validating a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for fosfomycin quantification in plasma samples. The significance lies in bridging the gap between analytical chemistry and clinical pharmacology, enabling personalized medicine approaches for patients receiving fosfomycin. The method's applicability in real-life clinical settings underscores its potential impact for routine TDM and improved therapeutic management. Experimental Design and Methodology The researchers developed an ultraperformance liquid chromatography-tandem mass spe