Enzyme Inhibitory Activities and RP-HPLC Analysis of Geranium and Erodium Species.

This study investigates enzyme inhibitory activities and rp-hplc analysis of geranium and erodium species.. The research utilizes high-performance liquid chromatography (HPLC) as the primary analytical technique to address specific challenges in pharmaceutical analysis. The genera Geranium and Erodium (Geraniaceae) have been documented to possess diverse ethnopharmacological uses, including diabetes mellitus. Relevant to their ethnopharmacological use, the current study aimed to evaluate the α-glucosidase, α-amylase, acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) enzyme inhibitory activity of ethanol extracts from 46 samples belonging to thirty-one species of... Research Background and Significance The study titled "Enzyme Inhibitory Activities and RP-HPLC Analysis of Geranium and Erodium Species" investigates the bioactive potential of ethanol extracts derived from thirty-one species belonging to the Geranium and Erodium genera, with a focus on their enzyme inhibitory properties. These genera, members of the Geraniaceae family, have been traditionally employed in ethnopharmacology, notably for ailments such as diabetes mellitus. The inhibition of enzymes like α-glucosidase and α-amylase is a well-established therapeutic approach for controlling postprandial hyperglycemia in diabetic patients, while acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition is relevant for neurodegenerative conditions such as Alzheimer's disease. By applying reversed-phase high-performance liquid chromatography (RP-HPLC), the study aims not only to quantify key phytochemicals but also to correlate their presence with enzyme inhibitory activity, underscoring the analytical and pharmacological significance of these species in pharmaceutical research. Experiment