Direct and simultaneous analysis of lipase-catalyzed hydrolysis of high-oleic oil model by chiral stationary phase HPLC-ELSD.

This study focuses on direct and simultaneous analysis of lipase-catalyzed hydrolysis of high-oleic oil model by chiral stationary phase hplc-elsd.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the food safety field. A novel HPLC-based method for direct separation of trioleoylglycerol (TOG), a major component in high-oleic oils, and its seven hydrolysis products (i.e., oleic acid, monooleoylglycerol (MOG) and dioleoylglycerol (DOG) isomers) was established using a chiral stationary phase column, Chiralpak IA. Within 20 min, all species including enantiomeric MOG (1-sn-MOG and... Research Background and Significance High-oleic oils, characterized by their elevated oleic acid content, are widely used in food industry applications due to their nutritional benefits and oxidative stability. Monitoring the enzymatic hydrolysis of these oils, particularly by lipases, is critical in food safety and quality control to understand lipid degradation pathways and to assess product authenticity and stability. Traditional analytical methods often face challenges in simultaneously quantifying complex mixtures of triglycerides and their hydrolysis products with high specificity and resolution. This study by Choi et al. (2022) addresses these challenges by developing a direct and simultaneous high-performance liquid chromatography (HPLC) method using a chiral stationary phase coupled with an evaporative light scattering detector (ELSD). The ability to separate not only the major triglyceride component, trioleoylglycerol (TOG), but also its seven hydrolysis products including isomeric monooleoylglycerols (MOG) and dioleoylglycerols (DOG) isomers, is significant for advancing analytical capabilities in food safety. Experi