[Development progress of stationary phase for supercritical fluid chromatography and related application in natural products].

This study focuses on [development progress of stationary phase for supercritical fluid chromatography and related application in natural products].. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the chemical field. Supercritical fluid chromatography (SFC) is an environment-friendly and efficient column chromatography technology that was developed to expand the application range of high performance liquid chromatography (HPLC) using a supercritical fluid as the mobile phase. A supercritical fluid has a temperature and pressure that are above the critical values as well... Research Background and Significance Supercritical fluid chromatography (SFC) has emerged as a powerful chromatographic technique that bridges the gap between gas chromatography (GC) and high-performance liquid chromatography (HPLC). Utilizing supercritical fluids, primarily carbon dioxide above its critical temperature and pressure, as the mobile phase, SFC offers unique advantages such as reduced solvent consumption, faster analysis times, and enhanced selectivity. The stationary phase plays a pivotal role in determining the separation efficiency and selectivity of SFC. Recent years have witnessed significant progress in the development of novel stationary phases tailored for SFC, especially for complex matrices like natural products, which often present challenges due to their structural diversity and polarity range. This study by Song et al. (2023) explores the advancement of stationary phases specifically designed for SFC applications in analyzing natural products, aiming to expand the applicability and effectiveness of this environmentally friendly technology in chemical analysis. Experimental Design and Methodology The research