Development of apigenin monoclonal antibodies and an immunoassay for the quantification of apigenin in celery.

This study focuses on development of apigenin monoclonal antibodies and an immunoassay for the quantification of apigenin in celery.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the biopharmaceutical field. Apigenin, a 4',5,7-trihydroxyflavone compound with diverse biological activities, is found abundantly in vegetables and fruits, particularly in celery. The identification and quantification of apigenin have become critical indicators for quality control in raw pharmaceuticals, finished drugs, and formulations. This study established an indirect competitive enzyme-linked immunosorbent assay (icELISA) based on... Research Background and Significance Apigenin, chemically known as 4',5,7-trihydroxyflavone, is a naturally occurring flavonoid with extensive biological activities including antioxidant, anti-inflammatory, and potential anticancer effects. It is predominantly found in various vegetables and fruits, with celery being one of the richest sources. Given its pharmacological importance, the accurate quantification of apigenin in plant materials and pharmaceutical formulations is essential for quality control, efficacy validation, and regulatory compliance. Traditional analytical methods have faced challenges such as specificity, sensitivity, and throughput limitations. This study addresses these challenges by integrating immunoassay techniques with high-performance liquid chromatography (HPLC), providing a robust and reliable approach for apigenin quantification in biopharmaceutical contexts. The development of specific monoclonal antibodies against apigenin further enhances assay specificity, enabling precise detection even in complex matrices like celery extracts. Experimental Design and Method