Development of a reliable method for determination of

This study focuses on development of a reliable method for determination of . The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the environmental field. This study outlines the development and optimization of an analytical method using Disposable Pipette Extraction (DPX) followed by high performance liquid chromatography-mass spectrometry (HPLC-MS) analysis to determine NAs in medicines. HPLC-MS analysis utilized a reversed-phase and positive mode electrospray ion source. DPX parameters were optimized through univariate and multivariate analyses,... Research Background and Significance Environmental analysis frequently demands highly sensitive, selective, and reproducible methods to detect trace-level contaminants in complex matrices. The development of reliable chromatographic methodologies is pivotal in advancing environmental monitoring, regulatory compliance, and pollution control. This study addresses these imperatives by focusing on the determination of target analytes—presumably NAs (Nucleic Acids or Naphthenic Acids, as the exact analyte is not specified)—using advanced high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS). The integration of Disposable Pipette Extraction (DPX) enhances sample preparation efficiency, minimizes matrix interferences, and improves analyte recovery, which are critical in environmental samples often characterized by diverse and challenging matrices. By optimizing the DPX parameters through both univariate and multivariate approaches, the study ensures robust extraction conditions, which directly impact the analytical sensitivity and reproducibility of the HPLC-MS method. The choice of reversed-phase chromatography coupled with positive mode elec