Determination of Oxaliplatin and Curcumin in Combination via Micellar HPLC and Its Method Validation.

This study focuses on determination of oxaliplatin and curcumin in combination via micellar hplc and its method validation.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the environmental field. A micellar-HPLC method was developed for the determination of oxaliplatin (OHP) and curcumin (CUR) employing a C18 column [4.6 × 250 mm, particle size (dp) = 5 µm] and diode array detector.... Research Background and Significance The simultaneous determination of oxaliplatin (OHP) and curcumin (CUR) presents analytical challenges due to their distinct chemical properties and environmental relevance. Oxaliplatin, a platinum-based chemotherapeutic agent, and curcumin, a natural polyphenolic compound with therapeutic potential, are increasingly studied in environmental matrices due to their presence as pharmaceutical contaminants and bioactive compounds. Accurate and reliable quantification of these compounds in combination is essential for environmental monitoring and pharmacokinetic studies, necessitating robust analytical techniques. Micellar High-Performance Liquid Chromatography (micellar-HPLC) offers a green and efficient alternative to conventional reversed-phase HPLC by employing surfactant-containing mobile phases, which enhance selectivity and reduce organic solvent consumption. This study’s significance lies in developing and validating a micellar-HPLC method tailored for OHP and CUR, addressing the need for environmentally friendly, precise, and reproducible analytical methods in environmental research. Experimental Design and Methodology The researchers employed a micellar-HPLC approach using a reversed-phase C18 column (4.6 × 250 mm, 5 µm particle size) coupled with a diode array detector (DAD) for s