Design-assisted HPLC-UV method for therapeutic drug monitoring of pholcodine, ephedrine, and guaifenesin in biological fluids.

This study focuses on design-assisted hplc-uv method for therapeutic drug monitoring of pholcodine, ephedrine, and guaifenesin in biological fluids.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the clinical field. Drug-drug interactions may amplify or diminish their intended effects, or even produce entirely new effects. Multicomponent mixture HPLC analysis offers a thorough and effective method for comprehending the makeup and behavior of complicated materials, advancing research and development across a range of scientific and industrial domains. A novel experimental design-assisted... Research Background and Significance Therapeutic drug monitoring (TDM) is a critical aspect of clinical pharmacology, especially for drugs with narrow therapeutic windows or significant pharmacodynamic variability. Pholcodine, ephedrine, and guaifenesin are commonly prescribed drugs used in respiratory conditions; however, their pharmacokinetics can be influenced by drug-drug interactions, which may alter therapeutic efficacy or induce adverse effects. Accurate, sensitive, and reliable quantification of these drugs in biological fluids is essential for optimizing therapy, avoiding toxicity, and improving patient outcomes. The development of robust analytical methods that can simultaneously quantify multicomponent drug mixtures in complex biological matrices remains a challenge in clinical laboratories. This study addresses this need by developing a design-assisted high-performance liquid chromatography coupled with ultraviolet detection (HPLC-UV) method specifically targeting the simultaneous quantification of pholcodine, ephedrine, and guaifenesin in biological fluids. The application of experimental design principle