Comprehensive characterization of the chemical composition of Lurong dabu decoction and its absorbed prototypes and metabolites in rat plasma using UHPLC-Q Exactive Orbitrap-HRMS.

This study focuses on comprehensive characterization of the chemical composition of lurong dabu decoction and its absorbed prototypes and metabolites in rat plasma using uhplc-q exactive orbitrap-hrms.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the pharmaceutical field. Lurong Dabu decoction (LRDBD) is an effective traditional Chinese Korean ethnic medicine prescription composed of eight herbs, which is used for treating asthma. However, its material basis has not been studied yet. Herein, the use of a new and highly sensitive UHPLC-Q Exactive Orbitrap-HRMS technique is proposed for the high-resolution... Research Background and Significance Lurong Dabu decoction (LRDBD) is a traditional Chinese Korean ethnic medicine prescription comprising eight herbs, historically used to treat asthma. Despite its long-standing clinical application, the chemical basis underpinning its therapeutic effects remained largely unexplored prior to this study. Comprehensive elucidation of the chemical constituents, including both the original compounds present in the decoction and their metabolites absorbed into systemic circulation, is critical for understanding the pharmacological mechanisms and ensuring quality control in pharmaceutical development. The study leverages ultra-high-performance liquid chromatography coupled with Q Exactive Orbitrap high-resolution mass spectrometry (UHPLC-Q Exactive Orbitrap-HRMS), a cutting-edge analytical technique that offers exceptional sensitivity, resolution, and accuracy. By applying this advanced methodology, the research addresses a crucial gap in the characterization of LRDBD, further advancing the integration of traditional medicines into modern pharmaceutical frameworks. Expe