Chromatographic authentication of botanical origin: Herbaceous pollen profiling with HPLC, HPTLC and GC-MS analysis.

This study focuses on chromatographic authentication of botanical origin: herbaceous pollen profiling with hplc, hptlc and gc-ms analysis.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the chemical field. This study describes a robust chromatographic authentication methodology for herbaceous pollen, employing gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC) and high-performance thin liquid chromatography (HPTLC) protocols. The comprehensive profiling of organic compounds not only distinguishes between different botanical sources but also establishes a reliable framework for quality control and... Research Background and Significance The authentication of botanical origin in herbaceous pollen samples is a critical task in both botanical research and quality control of natural products. As different plant species produce chemically distinct pollen profiles, accurate identification aids in biodiversity studies, environmental monitoring, and verifying the authenticity of pollen-derived products such as honey or herbal supplements. Traditional morphological methods for pollen identification, while useful, are often limited by subjective interpretation and overlapping characteristics. Chromatographic techniques, particularly High-Performance Liquid Chromatography (HPLC), offer a powerful analytical approach by providing detailed chemical fingerprints of pollen extracts. This allows for objective, reproducible, and high-throughput profiling of complex organic compounds. The study by Aziza et al. (2024) addresses the need for a robust chromatographic authentication method by combining HPLC with complementary chromatographic techniques, such as High-Performance Thin La