Chemical detection and analysis of Astragalus-Cassia twig drug pair using UHPLC-Q-TOF-MS and HPLC-UV methods.

This study focuses on chemical detection and analysis of astragalus-cassia twig drug pair using uhplc-q-tof-ms and hplc-uv methods.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the pharmaceutical field. The classic Astragalus-Cassia twig drug pair has a long history of proven efficacy. However, a fewer studies on material basis of the Astragalus and Cassia twig decoction (ACD) was researched at present. The method of UPLC-Q-TOF-MS for classifying and identifying the main chemical components of ACD was established and the... Research Background and Significance Astragalus-Cassia twig drug pair is a traditional herbal formulation with a longstanding history of therapeutic use in traditional medicine systems. Despite its widespread clinical efficacy, the comprehensive chemical characterization of this drug pair remains insufficiently explored, especially with regard to the material basis of the Astragalus and Cassia twig decoction (ACD). Accurate identification and quantification of the bioactive compounds are crucial for ensuring consistent quality, efficacy, and safety in pharmaceutical applications. The study leverages advanced chromatographic and mass spectrometric techniques to fill this knowledge gap, providing a robust analytical framework for the complex chemical matrix of ACD. The integration of ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) and high-performance liquid chromatography with ultraviolet detection (HPLC-UV) enables comprehensive profiling and quantification of constituents. This dual approach addresses the challenges posed by the complex herbal matrix, facilitating high-resolution separation and sensitive detect