Authenticity and quality evaluation of different Rhodiola species and commercial products based on NMR-spectroscopy and HPLC.

This study focuses on authenticity and quality evaluation of different rhodiola species and commercial products based on nmr-spectroscopy and hplc.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the chemical field. The main concern regarding the authenticity and quality of Rhodiola rosea L. (Sedum rosea (L.) Scop.) products is their adulteration with other Rhodiola species.... Research Background and Significance Rhodiola species, particularly Rhodiola rosea L., are widely recognized for their adaptogenic properties and therapeutic potential, leading to their increased commercial demand in herbal supplements and pharmaceutical formulations. However, the authenticity and quality of Rhodiola products have become a growing concern due to frequent adulteration with other Rhodiola species, which may differ significantly in their chemical profiles and bioactivity. Ensuring product authenticity is crucial not only for consumer safety but also for maintaining the integrity of biological efficacy claims. This study by Marchev et al. (2020) addresses the need for reliable analytical techniques capable of differentiating Rhodiola species and evaluating commercial product quality. The integration of nuclear magnetic resonance (NMR) spectroscopy and high-performance liquid chromatography (HPLC) offers a comprehensive approach to chemical characterization, enhancing the ability to detect adulteration and quantify key bioactive constituents. The investigation thus holds substantial significance for quality control in phytochemical analysis and supports regulatory compliance in the herbal products industry. Experimental Design and Methodology The research design employed a combination of NMR spectroscopy and HPLC to ac