Application of eco-friendly natural deep eutectic solvents (NADES) in HPLC for separation of complex natural products: Current limitations and future directions.

This study focuses on application of eco-friendly natural deep eutectic solvents (nades) in hplc for separation of complex natural products: current limitations and future directions.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the chemical field. Natural deep eutectic solvents (NADES) have been used in chromatography as extraction media and HPLC mobile phase additives, but only once have they been used as HPLC major mobile phase component. This review illustrates current knowledge and major limitations on use of NADES in HPLC mobile phase as well as... Research Background and Significance High-performance liquid chromatography (HPLC) remains a cornerstone analytical technique for the separation and quantification of complex natural products in chemical and pharmaceutical research. However, conventional organic solvents used in HPLC mobile phases often raise concerns regarding toxicity, environmental impact, and sustainability. The emergence of natural deep eutectic solvents (NADES) as greener, eco-friendly alternatives presents a promising avenue to address these challenges. NADES are formed by mixing natural primary metabolites such as sugars, organic acids, and amino acids, resulting in solvents with favorable physicochemical properties, including biodegradability and low toxicity. This review article by Heck et al. (2024) critically examines the application of NADES in HPLC, focusing on their role as extraction media and mobile phase additives, and importantly, explores the pioneering use of NADES as major components of HPLC mobile phases. The study is significant as it aligns with the growing demand for sustainable analytical practices while pushing the boundaries of chromatographic science to a