Anti-colorectal cancer actions of Glycyrrhiza uralensis Fisch. and its underlying mechanism via HPLC integration and network pharmacological approaches.

This study focuses on anti-colorectal cancer actions of glycyrrhiza uralensis fisch. and its underlying mechanism via hplc integration and network pharmacological approaches.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the pharmaceutical field. The therapeutic and prognostic outcomes for colorectal cancer (CRC) remain unsatisfactory. Among multiple reported bioactive functionalities of Glycyrrhiza uralensis Fisch. one vital recently reported activity is its therapeutic role against numerous cancers but limited information is available related to its underlying key mechanisms and therapeutically active ingredients, especially against CRC... Research Background and Significance Colorectal cancer (CRC) remains a leading cause of cancer-related morbidity and mortality worldwide, with current therapeutic and prognostic options still yielding limited success. Natural products have gained attention due to their multifaceted bioactivities and relatively favorable safety profiles. Glycyrrhiza uralensis Fisch., commonly known as Chinese licorice, has long been recognized in traditional medicine for a variety of pharmacological effects, including anti-inflammatory, antiviral, and anticancer properties. However, despite increasing reports on its anticancer potential, the detailed mechanisms underlying its effects on CRC and the identification of its active constituents have remained inadequately explored. This study addresses this gap by integrating high-performance liquid chromatography (HPLC) with network pharmacology to elucidate the anti-colorectal cancer actions of Glycyrrhiza uralensis Fisch. The use of HPLC not only enables the precise qualitative and quantitative analysis of bioactive compounds but also