Analysis of Methamphetamine in Urine by HPLC with Solid-Phase Dispersive Extraction and Solid-Phase Fluorescence Derivatization.

This study focuses on analysis of methamphetamine in urine by hplc with solid-phase dispersive extraction and solid-phase fluorescence derivatization.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the pharmaceutical field. We have developed a fluorescence detection-liquid chromatography (HPLC-FL) method that involves sample pretreatment by solid-phase dispersive extraction (SPDE) and solid-phase fluorescence derivatization for the simple and rapid analysis of methamphetamine (MA) in urine. This method uses a reversed-phase polymeric solid-phase gel to clean up analytes in SPDE, followed by fluorescence... Research Background and Significance Methamphetamine (MA) is a potent central nervous system stimulant with high abuse potential, and its detection in biological fluids such as urine is crucial for clinical toxicology, forensic investigations, and pharmaceutical monitoring. Accurate quantification of MA in urine presents analytical challenges due to its low concentration, matrix complexity, and interference from endogenous compounds. Traditional analytical methods often require extensive sample preparation, which can be time-consuming and prone to analyte loss. The study by Saito et al. (2023) addresses these challenges by developing a method combining solid-phase dispersive extraction (SPDE) with solid-phase fluorescence derivatization coupled with high-performance liquid chromatography (HPLC). This approach aims to provide a rapid, sensitive, and reliable technique for analyzing MA in urine, enhancing throughput and analytical confidence in pharmaceutical and forensic laboratories. Experimental Design and Methodology The methodology integrates innovative sample preparation techniques with robust chr