Analysis of authorized coccidiostats in chicken feces and environmental water by ultra-performance liquid chromatography-tandem mass spectrometry based on dispersive solid-phase extraction and lyophilization.

This study focuses on analysis of authorized coccidiostats in chicken feces and environmental water by ultra-performance liquid chromatography-tandem mass spectrometry based on dispersive solid-phase extraction and lyophilization.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the environmental field. A simple, sensitive, and efficient method based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the determination of 8 coccidiostats in chicken feces and environmental water (including sewage, pond water, and lake water) surrounding the farm. Target analytes in chicken feces were extracted with 2% acetic acid in... Research Background and Significance The monitoring of authorized coccidiostats in environmental matrices such as chicken feces and surrounding water bodies is essential for evaluating the impact of veterinary drug residues on ecosystems and public health. Coccidiostats are widely used in poultry farming to prevent coccidiosis, a parasitic disease affecting the intestinal tract of birds. However, their residues can enter the environment through fecal excretion and runoff, potentially contaminating water resources and soil. The accurate detection and quantification of these compounds at trace levels pose significant analytical challenges due to complex sample matrices and low analyte concentrations. Ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) offers enhanced sensitivity, selectivity, and speed, making it a powerful tool for such environmental analyses. This study by Ding et al. addresses these challenges by developing a robust, sensitive method for simultaneous determination of eight authorized cocci