[Analysis and evaluation on Paeoniae Radix Alba from different cultivars by UPLC-Q-TOF-MS and HPLC].

This study investigates [analysis and evaluation on paeoniae radix alba from different cultivars by uplc-q-tof-ms and hplc].. The research utilizes high-performance liquid chromatography (HPLC) as the primary analytical technique to address specific challenges in pharmaceutical analysis. In this study, an established ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS) method was combined with multivariate statistical analysis to investigate the commonality and difference of main chemical components in the medicinal parts of Paeonia lactiflora from different cultivars; in addition, a high performance liquid chromatography(HPLC) method was... Research Background and Significance Paeoniae Radix Alba, derived from the root of Paeonia lactiflora Pall., is a widely utilized traditional Chinese medicinal herb known for its diverse pharmacological activities, including anti-inflammatory, immunomodulatory, and analgesic effects. Given the increasing global demand for herbal medicines and the critical role of chemical consistency in ensuring therapeutic efficacy and safety, accurate qualitative and quantitative analysis of its bioactive constituents is essential. Different cultivars of Paeonia lactiflora exhibit variability in chemical composition, which can affect the pharmacological properties. This study addresses the significant need to comprehensively analyze and evaluate the chemical profiles of Paeoniae Radix Alba from various cultivars using advanced chromatographic and mass spectrometric techniques. Employing both ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and high-performance liquid chromatography (HPLC), the research aims to provid