A paradigm for sustainable analysis: QbD-driven HPLC-DAD for triple antiallergics in pharmaceutical, biological, and environmental samples.

This study focuses on a paradigm for sustainable analysis: qbd-driven hplc-dad for triple antiallergics in pharmaceutical, biological, and environmental samples.. The research employs high-performance liquid chromatography (HPLC) techniques to address analytical challenges in the environmental field. This study introduces an innovative approach combining Quality by Design (QbD) principles with high-performance liquid chromatography and diode array detection (HPLC-DAD) for the simultaneous analysis of tranilast (TNL), pranoprofen (PPF), and chlorpheniramine maleate (CPM), a novel antiallergic combination therapy. The QbD framework was systematically employed to optimize critical method parameters,... Research Background and Significance The increasing prevalence of environmental contamination by pharmaceutical residues has become a major concern, necessitating the development of robust analytical methodologies for their detection and quantification. Among these contaminants, antiallergic drugs such as tranilast (TNL), pranoprofen (PPF), and chlorpheniramine maleate (CPM) are frequently detected in environmental matrices due to their widespread usage. Traditional analytical methods often face challenges related to sensitivity, selectivity, and environmental sustainability. This study presents a paradigm shift by integrating Quality by Design (QbD) principles with high-performance liquid chromatography equipped with diode array detection (HPLC-DAD) to develop a sustainable, reliable, and efficient method for simultaneous quantification of these three antiallergics in pharmaceutical, biological, and environmental samples. The significance lies not only in the analytical performance but also in the method’s sustainability, aligning with green analytical chemistry principles